KMID : 1147720090020040288
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Journal of Acupuncture and Meridian Studies 2009 Volume.2 No. 4 p.288 ~ p.293
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In Vitro Cholinomimetic Effect of Loranthus Ferrugineus in Isolated Guinea Pig Ileum
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Ameer Omar Ziad
Salman Ibrahim M. Siddiqui Mohammad Jamshed Ahmad Yam Mun Fei Sriramaneni Raghava Naidu Sadikun Amirin Ismail Zhari Shah Amin Malik Asmawi Mohd. Zaini
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Abstract
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This study aimed to elucidate the mechanism(s) of the spasmogenic action of Loranthus ferrugineus in isolated guinea pig ileum. Thus the contractile responses of guinea pig ileum to graded additions of either L. ferrugineus methanol extract or its n-butanol fraction were tested in the presence and absence of various pharmacological interventions. The data showed that L. ferrugineus methanol extract and the n-butanol fraction produced a concentration-dependent spasmogenic effect in isolated guinea pig ileum segments. These effects were significantly inhibited in the presence of 1 ¥ìM atropine. In contrast, the response to the lowest concentrations of L. ferrugineus methanol extract (0.25, 0.5 and 1 mg/mL) and n-butanol fraction of L. ferrugineus (0.125, 0.25 and 0.5 mg/mL) were considerably enhanced in the presence of 0.05 ¥ìM neostigmine. Neither L. ferrugineus methanol extract nor n-butanol fraction contractile responses were affected upon the incubation of the ileal segments with 100 ¥ìM hexamethonium. The results of this study show that the spasmogenic effect of L. ferrugineus is possibly mediated through a direct action on intestinal muscarinic receptors. It is suggested that the bioactive constituents of L. ferrugineus serve as a substrate for acetylcholinesterase.
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KEYWORD
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acetylcholine, acetylcholinesterase, cholinergic activity, guinea pig ileum, Loranthus ferrugineus, muscarinic receptors
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